| Two novel human genes were isolated by homologous cloning strategy in our lab, and were named as HNl and HNl like gene respectively due to their homolog with mouse gene Hn1 (hematological and nerological expressed sequence 1).HNl localizes on human chromomosome 17q25.2, composed of five exons and four introns. The 1621 bp cDNA of HNl have an open reading frame encoding a protein of 154 aa. HNl have two transcripts of 1.2 and 1.6-kb both expressed highly in testis and skeletal muscle indicated by Northern blot. By EST data analysis, we analized two transcripts of HNl are caused by different poly(A) tailing. HNl produces a 18.5 KDa protein when expressed in different cells lines, and its fusion protein with GFP localize in nucleus in HeLa cells. By EST data analysis and specific primer amplification, we proved that HNl can produce three different splicing transcript in human tissues including liver, brain etc. HNl can activate pathway associated mainly with cell proliferation and differiation in HEK293T cells by Luciferase report gene assay. Focus cells which expressed stably HNl have retardant S phase of cell cycle by Flow analysis. Human liver cDNA library was screened to find the interacting molecular through yeast two hybridization using HNl as bait, a FAS associated factor (FAF1) was found to interact with HNl in yeast. We confirmed their interaction by co-immunopricipitation and co-localization in vivo. It is proposed that HNl may be involved in FAS induced apoptosis processing.HNIL localize on human chromosomal band 16p13.3, composed of five exons and four introns. The 3046 bp cDNA of HNIL contains an open reading frame encoding 190 aa. HNl transcribes three bands of 4.2, 4 and 2-kb which are transcibed widely. Based their sequence, we conclude that the cDNA cloned in our lab represents 4-kb isoform, the alternative poly(A) tailing cause 2-kb isoform, another exon splicing new transcript cause the 4.2-kb form, and we confimed 6-exons transcript by PCR amplification in human tissues including liver. HNIL produce a 20.5 KDa protein when expressed in cells, its protein localizes in both cytoplasm and nucleus. HNIL activates the pathway associated mainly with cell cycles when expressed in 293T cells. It is reported that HNIL is often involved in chromosome deletion of 16p in several human diseases, and the ESTs of HNIL are derived mostly from tumor tissues. It was supposed that HNIL may be associated with these disorder.Further, we predicted and identified 14 HNl otholog genes, 20 HNIL orthologgenes and 9 pseudogenes of them. By multiple sequence alignment and phylogenetic tree, we discussed that HNl and HN1L have the common genesis in evolution process. HNl exists in species of vertebrates, HNl of human, chimpanzee and mucaca have the same protein sequence. HN1L has homolog sequence in vertebrates, invertebrates, plants even yeast, and HN1L of mouse and rat have two putative sequences. Motif predition and analysis indicate that the location of two putative motifs in HN1 group is contrary with that in HN1L group. Base on these facts, we define them as an novel gene family, HN1 gene family members are highly conserved...
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