A Putative Plasmodesmata-associated Protein,At1g1190,in Arabidopsis Thaliana

Pds (plasmodesmata) are channels that traverse the cell wall to establish a symplastic continuum through most of the plant. As we known, AtRGP2is a plasmodesmal associated protein delivered to plasmodesmata via the Golgi apparatus and stained callose using aniline blue as another plasmodesmal marker (Sagi et al2005). In this paper, we construct GFP:At1g19190fusion protein, compare with AtRGP2:YFP and use callose as marker, found At1g19190could target to Pd. To test the localization of At1g19190with cytoskeleton, we use cell skeleton disturb drugs treated with stably expressed GFP: At1g19190transgenic Arabidopsis seeding, our research showed colchicine could disturb At1g19190located to Pd. Cytochalasin B (CB) and BFA do not affect the Pds-localization of GFP:At1g19190.We use micrografting methods to study long distance transport of At1g19190and AtRGP2, to our surprise, we found when graft stably expressed GFP:At1g19190transgenic Arabidopsis with wild-type (WT), fluorescence could test in no-fluorescence parts (wild-type (WT)). If we graft expressed AtRGP2:YFP transgenic Arabidopsis with WT, AtRGP2:YFP fluorescence could not transport to no-fluorescence parts. In conclusion, At1g19190is a plasmodesmal associated protein, and At1g19190may be a very important protein in the Pds structure and may play a critical role in substance transport. Pd-localization of At1g19190is dependent on MTs. It could help us to know the mechanism of long distance transport.