Study On The Expression And Function Of Zinc Transporter ZnT3, ZIP4 And LIV1

Study on the expression and function of zinc transporter ZnT3, ZIP4 and LIV1 Abstract: To observe the expression and function of ZnT3, ZIP4 and LIV1, the zinc and ZnT3 distribution, the effects of zinc on cell growth and zinc transporters expression and the effects of LIV1 in EMT in breast cancer were investigated. The results showed that the coincident distribuition of zinc and ZnT3 mRNA in mouse brain that their expression were higher in cerebral cortex and hippocampus. Contrast to the unchanged ZIP1 expression, the MT1, ZnT1 and ZIP4 mRNA expression were in a dose- and time-dependent manner. The DMT1 mRNA expression was time-dependently upregulated but not concentration-dependently in the late TPEN treatment duration. The silence of LIV1 could downregulate the expression of E-cadherin and promoted the cell growth significantly. Overexpression vector acted the contrary effects. The results suggested that the high zinc content in cerebral cortex and hippocampus might be determined by the high expression of ZnT3 mRNA in these areas. In addition, it is proposed that, in response to variations in zinc concentration, the cooperated regulative roles of ZnT1, MT1, DMT1, ZIP4 provide foundational contribution to keeping cellular zinc homeostasis. The zinc transporter LIV1 can regulate the EMT via the regulation of the E-cadherin expression, which suggests that zinc transporter LIV1 play a role in EMT in breast cancer as a antioncogene.