Study Of The Expression Profile Of KDR In The Prostatic Carcinoma And Its Role In PC-3 Cell Line

Objective: Prostate carcinoma has a high incidence and death rate in man in the west country, and these data also have a upgrade tendency in Asia. Even though we have many ways to heal it, but we have not an utility way to cure androgen independence prostatic cancer, so it is become more important to find ways to inhibite cancer cell growth or induce apoptosis. We have found that VEGF has close correlation with the happen and development of cancer. There are many reports of how dose VEGF work in the prostatic carcinoma, but there are few works about the relationship of KDR and the PCa. So, I want to study the expression profile of KDR in the three prostatic carcinoma cell lines, and the expression of KDR in the prostatic carcinoma and benign prostate hypertrophy tissues. To transfecte different concentration of KDR antisense oligodeoxynucleotide in PC-3 cell, we detecte the effect of the expression of KDR, and study the inhibition rate of PC-3 cell ,the cell cycle and the apoptosis.Methods: Culturing the cell line of LNCap, DU-145 and PC-3. We used real time PCR to detect the expression profile of KDR in the three cell line. The immunohistochemistry was used to detect the expression of KDR in 48 prostatic carcinoma and 20 benign prostate hypertrophy tissues. After transfected antisense oligodeoxynucleotide in PC-3 cell, which express KDR more strong,immunohistochemistry and real time PCR were once again carried out to detect the expression profile of KDR in the cell. The growth inhibition of PC-3 cell was observed by MTT, and the cell cycle and apoptosis were analyzed by FCM.Results We found that KDR expressed in prostate carcinoma and benign prostate hypertrophy tissues, but the expression of KDR was stronger in prostate adenocarcinoma than in BPH tissues, and there was no relationship between histological grades of carcinoma.The real time PCR displayed that KDR expressed most strong in PC-3 cell ,and the weakest in LNCap cell. The immunohistochemistry told us 48 hours later after transfected antisense oligodeoxynucleotide in PC-3 cell, the cell growth inhibition was the strongest.That caused a lower survival rote of PC-3 cell, and the inhibition was positive correlation with the concentration of KDR antisense oligodeoxynucleotide. Sense oligodeoxynuleotide and Lipofectamine₂₀₀₀ could not inhibit the cell growth. The antisense oligodeoxynucleotide depressed the KDR mRNA of the PC-3 cell line. The FCM displaied that the cell cycle had no change, but different level apoptosis displaled in different concentration, and the highest inhibition was 48.6%.Conclusion KDR expressed in prostatic carcinoma and benign prostate hypertrophy tissues. The expression of KDR in prostatic carcinoma tissues were stronger than that in benign prostate hypertrophy tissues, and there has no different between the different Gleason's score. All the three prostatic carcinoma cell line expressed KDR, the expresson of KDR is strongest in PC-3 cell line and the weakest in LNCap cell line. Different concentration of KDR antisense oligodeoxynucleotide lowered dowm the KDR expression in different level, and caused different level cell growth inhibition and apoptosis. It has no effection in the cell cycle, perhaps it told us that the KDR antisense oligodeoxynucleotide can cause apoptosis in every period cell.