Eukaryotic Expression And Polyclonal Antibody Preparation Of The Artificial Composite N-terminal Gene Of SHH

AIM:To get the eucaryonic recombinate expression of sonic hedgehog in hedgehog signal path in human pancreatic cancer,and prepare the polyclonal antibody of SHH.Methods: Construct the eukaryotic expression vector of the SHH-N,get the pichia pastoris expression strain, to induce the destination protein by using the methanol. Immune the Balb/c mouse to have the polyclonal antibody of SHH.Results: The eukaryotic expression vector of the SHH-N was constructed successfully,get the pichia pastoris expression strain and the destination protein. Prepare the polyclonal antibody of SHH with high quality.Conclusion:Recombinant plasmid vector of SHH-N was constructed and expressed protiein successfully.The SHH polyclonal antibody was made,which provide a useful tool in the further study of Hedgehog signal path in human pancreatic cancer.