Effect Of Red Peony Root Decoction For Severe Acute Pancreatitis And For Inflammatory Mediators In Rats

Objective: To observe the therapeutic effect of red peony root decoction (RPRD) in treating SAP, and the effect of it on the expression of NF-κB, TNF-αand IL-6. Methods: Sixty patients with SAP were randomly divided into two groups. The control group (n=30) was treated with routine treatment and the treatment group (n=30) was treated with routine treatment and RPRD. SAP was induced by intraperitoneal injection of L-arginine and was interfered by RPRD/paeoniflorin. Rats of each group were sacrificed and blood samples were taken for the measurement of serum amylase, serum TNF-αand IL-6. The expression of NF-κB p65 protein and TNF-αand IL-6 mRNA in the pancreas were detected by immunohistochemistry, Western Blot and fluorescent quantification PCR (FQ-PCR), respectively, and pathological studies were analyzed. Results: RPRD could improve quickly symptom, laboratory criteria, and reduce significantly the complication incidence rate. RPRD/paeoniflorin can significantly improve pancreatic injury, decrease the mortality of SAP rats. RPRD/paeoniflorin had an inhibitory effect on the NF-κB activity and the level of TNF-αand IL-6 of the pancreatic tissue in SAP rats. Conclusion: RPRD has significant therapeutic effect in treating SAP and its mechanisms may be related to the inhibition of the activity of NF-κB and the down-regulation of the cytokines in the body.