| Backgroud: Benign prostatic hyperplasia(BPH) is a common and frequently occurringdisease in middle and older male. While the aging population in our country increasinggradually, the incidence of BPH also increases correspondingly, BPH has alreadyaffected the health and the quality of life.Objectives: To probe into the Traditional chinese medical(TCM) Pathologensis ofBPH. To investigate the mechanism of Kangquan Recipe(KQR) on BPH and observe clinicaleffect in ordor to provide data for Traditional Chinese Medical and Pharmacy on BPH.Methods:(1)animal experiment: 72 Sprague-Dawley male rats were randomly dividedinto six groups:normal group, model group, finasteride group, KQR low-dosage group,KQR medium-dosage group, KQR large-dosage group. Except for normal group, BPH modelrats of the rest groups were established by subcutaneously injecting testosteronepropionate after being castrated. The rats were killed and prostate glands wereremoved after intragastric administration respectively for 30 days.Theweight, volume and index of the prostate were measured. The pathomorphological changeswere examined under lightmicroscope. By means of enzyme linked immunosorbent assay(ELISA),the plasma levels of testosterone(T) and estradiol(E2) were examined. Bymeans of immunohistochemisty, the expressions of Ki-67 and bFGF were examined in theprostates of rats. By means of RT-PCR, the expressions of BaxmRNA, Bcl-2mRNA, PCNAmRNAand bFGFmRNA were examined in the prostates of rats. (2)clinical study:60 middle and older male cases of BPH were randomly divided into two groups: KQR groug andFinasteride group. The cases were treated respectively by KQR and Finasteride for12 weeks. Changes of international prostate symptom scores (I-PSS), quality oflife(QOL), TCM symptomatic scores, clinical symptoms, maximun flow rate(Qmax), averageflow rate(Qave), residual urine volume(RUV), total prostatic volume (TPV) in patientswith BPH were observed before and after treatment.Results:(1)animal experiment:①Compared with model group, prostaticweights, volumes and indexs decreased significantly in KQR large, medium-dosagegroups (P＜0.05 or P＜0.01) and prostatic weights decreased significantly in KQRlow-dosage group (P＜0.05).②Model rats had obvious anomaly in pathomorphology,KQR can restrain the hyperplasia of prostate.③Compared with model group, the levelsof testosterone decreased significantly in all KQR treatment groups (P＜0.05 orP＜0.01), the levels of estradiol increased significantly in KQR large, medium-dosagegroup (P＜0.05), the ratios of E2/T increased significantly in all KQR treatment groups(P＜0.05 or P＜0.01).④Compared with model group, the expressions of BaxmRNA andratios of Bax/Bcl-2 increased significantly, the expressions of Bcl-2mRNA decreasedsignificantly in all KQR treatment groups (P＜0.01).⑤Compared with model group,the expressions of Ki-67 and PCNAmRNA decreased significantly in all KQR treatmentgroups (P＜0.05 or P＜0.01).⑥Compared with model group, the expressions of bFGFand bFGFmRNA decreased in all KQR treatment groups, the expressions of bFGF andbFGFmRNA decreased significantly in KQR large, medium-dosage groups (P＜0.05 orP＜0.01), but the expressions of bFGF and bFGFmRNA in KQR low-dosage group had nosignificantly differece (P＞0.05). (2)clinical study:①After treatment for 12weeks, the levels of I-PSS and QOL in patients with BPH were reduced significantlyin the treated group by KQR and the control group by finasteride (P＜0.01).In respect of reducing the levels of I-PSS and QOL, KQR and finasteride had no significantlydifference (P＞0.05). KQR reduced significantly the levels of TCM symptomatic scores(P＜0.05), finasteride reduced the levels of TCM symptomatic scores but notsignificantly (P＞0.05), KQR reduced the levels of TCM symptomatic scores superiorto finasteride (P＜0.05)②KQR and finasteride ameliorated significantly the symptomsof imcomplete emptying, frequency, intermittency, urgency, weak stream, hesitancy andnocturia (P＜0.05 or P＜0.01),KQR ameliorated the symptoms of urgency and nocturiasuperior to finasteride (P＜0.05), finasteride ameliorated the symptoms of imcompleteemptying superior to KQR (P＜0.05).③KQR and finasteride improved significantly thelevels of Qmax and Qave, KQR improved the levels of Qmax superior to finasteride(P＜0.05).In respect of improving the levels of Qave, KQR and finasteride had nosignificantly difference (P＞0.05).④KQR and finasteride reduced significantly thelevels of URV and TPV. In respect of reducing the levels of URV, KQR and finasteridehad no significantly difference (P＞0.05).Finasteride decreased the levels of TPVsuperior to KQR (P＜0.05).⑤The markedly effective rate, total effective rate inthe treated group was 33.3%,80% respectively. The markedly effective rate, totaleffective rate in the control group was 26.7%,76.7% respectively. The curativeeffects of both groups had no significantly difference (P＞0.05).⑥The markedlyeffective rate, total effective rate in the group of stagnation of blood stasis was38.9%,83.3% respectively. The markedly effective rate, total effective rate in thegroup of decline of kidney-Yang was 25%,75% respectively. The curative effects ofthe groups of stagnation of blood stasis and decline of kidney-Yang had nosignificantly difference in the treated group (P＞0.05).Conclusion:①The study has suggested that kidney weak is the fundamentalreason, blood stasis is the important pathological change, interior retention of heat and toxin is the main concurrent Symptom, prolonged illness obstructing thecollaterals is the important pathogenesis of BPH.②KQR shows multiway andmultitarget treatment effects on benign prostatic hyperplasia in experimental rats.KQR can effectively decrease prostatic weights, volumes and indexs, restrain thepathological hyperplasia of prostate, regulate the balance of plasma estradiol andtestosterone, regulate the expressions of baxmRNA and bcl-2mRNA so that they canpromote the cell apoptosis of prostate, decrease the expressions of Ki-67 and PCNAmRNAso that they can restrain the cell proliferation of prostate, decrease theexpressions of bFGF and bFGFmRNA in prostate of experimental rats.③KQR caneffectively reduce the levels of I-PSS and ameliorate quality of life, reduce thelevels of TCM symptomatic scores, ameliorated the symptoms of imcompleteemptying, frequency, intermittency, weak stream, urgency, hesitancy andnocturia, improve the levels of Qmax and Qave, reduce the levels of URV and TPV.④KQR promote the cell apoptosis superior to finasteride, but finasteride restrainthe cell proliferation and the expressions of bFGF superior to KQR in the prostatesof experimental rats. KQR reduce the levels of TCM symptomatic scores, improve thelevels of Qmax, ameliorate the symptoms of weak stream and nocturia superior tofinasteride, but finasteride reduce the levels of TPV, ameliorate the symptom ofimcomplete emptying superior to KQR in patients with BPH. above shows that KQR hasits characteristic and good prospects on BPH.Subjective headings: benign prostatic hyperplasia/tcd ther, cell apoptosis/drugeff, cell proliferation/drug elf, growth factor/drug eff, rat, male, @ KangquanRecipe...
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