Foundational Research On Phlegm-dampness Constitution And Correlation Between Phlegm-dampness Constitution And Metabolic Syndrome

Objective:To excavate the related theories of phlegm-dampness constitution from Traditional Chinese Medicine theory,summarize contemporary research achievement and expatiate the theories of phlegm-dampness constitution and its application.to explore the characteristics of single nucleotide polymorphisms(SNP)and copy number variation(CNV)of phlegm-dampness constitution,analyze the related gene function,expatiate the molecule mechanism. Develop correlation researchs between phlegm-dampness constitution and metabolic syndrome(MS) to prove the morbility proneness of phlegm-dampness constitution and provide theory basis to prevent these diseases early.Methods:By the hods of the traditional literature research and the contemporary literature analysis,we discuss the correlative theories of phlegm-dampness constitution from origin to development , from connotation to extension. by summarizing the clinical experience,this article discusses the application of phlegm-dampness constitution theory in preventing and curing disease. This subject uses Affymetrix GeneChip Mapping 500K Array to explore the characteristics of SNP and CNV of phlegm-dampness constitution. Utilize clinical epidemiology and medical statistics to analyze the correlation between phlegm-dampness constitution and MS.Results:It has been a long time for the traditional literature of TCM to the cognition of phlegm-dampness constitution. From the research,we consider that phlegm-dampness constitution is one of the common types of constitution which is affected by congenital heredity and acquired predisposition,and it can form certain body structure,physiological function and mental profile , and be prone to suffering from correlative diseases. Phlegm-dampness constitution can be regulated,which is the effective measure to prevent and cure correlative diseases.Examining the genome DNA of peripheral blood cells of six phlegm-dampness constitution and six normal constitution by using Affymetrix GeneChip Mapping 500K Array,15 CNVs and 6 SNPs with significant differences were identified between the phlegm-dampness constitution and the normal constitution. Further biological theme analysis on the genes identified between two constitution groups demonstrate that they are involve in enzyme activity, sterol transporter activity,olfactory receptor and binding protein, participate the biology process of the carbohydrate metabolism, gluconeogenesis, glycolytic pathway, lipid metabolism, cholesterol metabolic process,brown fat cell differentiation and proteolysis.We have proved a correlation between phlegm-dampness constitution and MS through the study of 3000 individuals via bivariate correlate.We detected the phlegm-dampness constitution is the most dangerous constitution factor of MS by the case-control study. Persons of phlegm-dampness constitution have higher level on body mass index(BMI), waistline(WC), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose(FBG), Fasting insulin(Fins),uric acid(UA) and have lower level on high-density lipoprotein cholesterol(HDL-C), adiponectin compared with those of normal constitution. There was significant difference between two groups(P<0.05).Conclusions : Theory study indicate: Phlegm-dampness constitution is a biased constitution state whose characteristic is stagnation of phlegm and dampness due to the dysfunction of transportation. Its formation is related to congenital heredity and acquired predisposition. The developing tendency of phlegm-dampness constitution is the stagnation of phlegm and dampness,and phlegm complicated with stasis,that lead to vertigo,apoplexy,thoracic obstruction and diabetes gradually. Invigorating the spleen,disinhibiting dampness,resolving phlegm,promoting the flow of qi,exercise and diet cure are the main methods for regulating phlegm-dampness constitution.Phlegm-dampness constitution is one of the types of constitution which is related to polygene,and has its unique SNP and CNV profile comparing with normal constitution persons. function of genes identified manifests that the gene characteristic of phlegm-dampness constitution is related to metabolism disorder.There is a relationship between phlegm-dampness constitutions and MS. Our results revealed persons of phlegm-dampness constitution have dysfuctions in lipid metabolism, glycometabolism and purine metabolism, they are high risk group of MS. Research on phlegm-dampness constitution is a way to investigate pathomechanism, prophylaxis and treatment of MS.