Effect Of Neovascularization And Glycosylation Of Ceramide On Malignant Transformation From Benign Nasal Inverted Papilloma To Carcinoma

Objective To investigate effect of neovascularization and glycosylation of ceramide on malignant transformation from precancerous lesion, benign nasal inverted papilloma (NIP) to cancer.Methods We detected the expression of CD34 ceramide and glucosylceramide synthase (GCS)in the paraffin samples of precancerous lesion NIP and malignant transformation tissues using immunohistochemistry (Envison method). Then the microvessel number of per unit area (MVNA) were measured and the optical density (OD) of ceramide and GCS were quantitatively analysed using computer image software. The NIP malignant transformation tissues were mainly compared with their precancerous lesion before, and single attacked NIP and recurrence NIP as control groups.Results There were precancerous lesion NIP group (n = 8) and NIP wi th carcinoma group( n = 25 ). We mainly compared the NIP malignant transformation tissues (n = 14) and their precancero.us lesion before (n = 8), and NIP with single at tack ( n = 10 ) group and NIP with recurrence ( n = 7 ) group as control groups.1 MVNASignificant increase of MVNA was observed in NIP with carcinoma (142.73± 32.77) (/mm~2) compared to other three groups (P < 0.05);Significant increase of MVNA was observed in precancerous lesion NIP (103.07 ± 8.04 ) ( /mm~2) compared to NIP with recurrence(68.52 ± 13.58)(/mm~2)and NIP with single attack (51.79±20.58) (/mm~2) ( P < 0. 05 );The MVNA in NIP with recurrence was higher than NIP with single attack, but the difference has no significance ( P> 0. 05 );Significant increase of MVNA was observed in NIP malignant transformation tissues ( 162. 56 ± 21. 01 ) ( /mm~2) and their precancerous lesion before ( 103. 07 ± 8. 04 )( /mm~2), ( t test, P = 0. 000)2. the optical density (OD) value of ceramideSignificant decrease of OD value of ceramide was observed in NIP with carcinoma (7. 50±2. 36 ) compared to other three groups (P < 0.05);Significant decrease of OD value of ceramide was observed in precancerous lesion NIP ( 10.85 + 1. 60 ) compared to NIP wi th recurrence ( 13. 86 ± 1. 02 ) and NIP wi th single attack ( 14. 84 ± 1. 69 ) ( P < 0. 05 );The OD value of ceramide in NIP with recurrence was lower than NIP with single attack, but the difference has no significance ( P> 0. 05 );Significant decrease of OD value of ceramide was observed in NIP malignant transformation tissues (6.47 ± 1. 23 ) and their precancerous lesion before ( 10. 85 ± 1. 60 ) ( t test, P = 0. 000) .3 the optical density (OD) value of GCSSignificant increase of OD value of GCS was observed in NIP with carcinoma (15.46 ± 2.70, n = 7) compared to precancerous lesion NIP ( 11. 30 ± 1. 36, n = 6) and NIP with single attack ( 10. 85 ± 1. 19, n = 4) (P < 0.05);The OD value of GCS in precancerous lesion NIP was higher than NIP with single attack, but the difference has no significance ( P > 0. 05 );Significant increase of OD value of GCS was observed in NIP malignant transformation tissues (15. 73±2. 85, n = 6) and their precancerous lesion before (11. 30 ±1.36, n = 6) ( t test, P = 0. 000) .In a word, there were significant increase of MVNA. decrease of ceramide and increase of GCS in NIP with carcinoma compared to precancerous lesion NIP; significant increase of MVNA, decrease of ceramide in precancerous lesion compared to NIP with recurrence and NIP with single attack.Conclusions Neovascularization and glycosylation of ceramide may play a significant role in the process of malignant transformation from benign NIP to carcinoma. The increase of MVNA, decrease of ceramide expression and increase of GCS expression could be clinical indicators of NIP malignant tendency.