Effect Of Proliferation And MCM3 Gene Expression In Osteoblasts Exposed To Fluoride

Objective: Fluorosis still is very severe in our country, it has many types and spreads widely, numbers of people who face to it are great. With more related research work being done, to explore the mole-mechanism of fluorosis with new techniques and skills of preclinical medical science can provide new path for the prevention of fluorosis. Based on the previous project, we choose MCM3 (minichromosome maintenance deficient 3) which is one of related genes of skeletal fluorosis to do further study. MCM3 is a kind of gene which has close relationship with DNA duplication, it's quantity and distribution in cells reflect cell's growth. After primary culture osteoblasts in mice being identified and improved, our project checks the concentration and distribution of fluoride ions in osteoblasts when osteoblasts are exposed different doses of fluoride with different period; observes the proliferation of osteoblasts exposed to fluoride in mice; detects MCM3 protein expression level, distribution and quantity of MCM3 mRNA of osteoblasts exposed to fluoride.Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing. (2) We observed the fluoride ions concentration and distribution in osteoblasts exposed to fluoride in mice by Nuclear Magenetic Resonance(NMR). (3)There were 5 experimental groups according to doses: 0, 5, 10, 20, 40mg/L. We observed the...