| Arisaematis is sub-class to the family of Rhizoma Arisaematis and branch of Rhizoma Arisaematis. As perennial grass plant, its tuber could be an effective components of medicine. It has been proved that has some medical therapeutic uses for clinical treatment of cough, hemiparalysis,tetanus and so on from ancient time. Arisaema amurense Maxim. was studied as a exprimental subject in this article. This plant distributes all around of Mountain of Changbai and some other rural area in Jilin Province. It has recorded much more.in application of anti-inflammation, while, the researches concerning with anti-tumor and its mechanisms on the molecular level has been less noted. Especially for those studies on transplanted hepatoma and in vitro inhibition test of hepatoma cells proliferation. For recent years, our team has been studying focus on pharmacodynamics of Arisaematis ingredients to treat anti liver cancer. Starting from inducing apoptosis of hepatoma cells, the effects on relative signal transduction pathway would be being studied.To evaluate the efficacy of Arisaematis ingredients anti human hematoma cells in vitro and observe its biofunction concerns with inducing apoptosis of cancer cells at all, we applied the following approaches to study.Firstly, MTT toxic experiment was used as to determined diminish and inhibition rate of human SMMC-7721 and Hep G2cultured in vitro after administrated with the various concentration of Arisaematis ingredients . Then, cytometry and DNA fragment electrophroresis were applied to analyze apoptosis of hepatoma cells. The capability of immune response was studied by means of lymphocyte transformation experiments. Laser Confocal was a precised method to used as detecting expression of Fas receptor which distributed on the surface of the membrane.The expression of Caspase-3 inside of rat liver tissue was measured by means of cytometry. Finally, Caspase-3, Bac-2/Bax transcription and expression in SMMCA-7721 being treated with Arisaematis ingredients was half quantitively determined by using of RT-PCR and western blotting on the level of nucleic acid and protein.The results show that.SMMC-7721 and HepG2, human hepatoma cancer cells, has been strongerly inhibited by Arisaematis ingredients with various concentration(2mg/ml, 4mg/ml, 8mg/ml, P<0.05 or P<0.001) compared with control group. Especially, at the concentration of 4mg/ml 8mg/ml, SMMC-7721 and HepG2 cells were remarkably inhibited at the time of 48h(P<0.001). In addition, typical apoptosis peak was found at these two concentration after administrating to SMMC-7721 cells. DNA fragment ladder was obviously observed at the middle and high concentration as well. It has been suggested that the inhibition of cancer cells concerns with induction of apoptosis. Compared with control group, Fas protein expression has been detected significant at the middle and high concentration( P<0.05) by means of density scanning. Caspase-3 expression expression at the middle and high concentration( P<0.05) are higher than that of control group. On the molecular level, Caspase-3 and Bax expression at various exprimental groups are significantly higher than that of the control group( P<0.05), meanwhile lower in Bcl-2 notably( P<0.05).Conclusion: Arisaematis ingredients shows signifant inhibition of growth human liver cancer cells strains such as SMMC-7721 and HepG2. This inhibition is strongly related with induction of apoptosis of cancer cells. Its mechanisms concern with triggering apoptosis pathway of Fas, caspase-3. This article supplis basic reseaching data for application in clinics further and reveal that having potencial advenatages in developing as a new drugs.
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