The Effects Of Fluoride On Marrow Stromal Cells, Their Products OPG, RANKL, And Related Cytokines

Osteoblast is one of the most important cells involved in the pathogenesis of skeletal fluorosis. Marrow stromal cells(MSCs),the precursors of osteoblasts ,should be affected by fluoride and play a role in the skeletal fluorosis. In this experiment,the objective is the effects of fluoride on MSCs and two cytokines,osteoprotegerin (OPG)/receptor activator of nuclear factorκB ligand(RANKL),which are produced by MSCs and involved in bone metabolism.Firstly,the fluorosis model of rats was established by ingesting excess fluoride(F-50,100mg/L in drinking water) with low calcium diet or not for 4 or 8 months .Secondly, the levels of OPG,RANKL,IL-1? and TNFαin serum,bone marrow and spleen were measured by methods of Elisa,IHC and RT-PCR respectively. Lastly, cultured MSCs were treated by different concentrations of fluoride in osteogenic medium or not . Methods ,involving of morphology ,MTT, Elisa ,ICC ,and RT-PCR ,were employed to observe the changes of MSCs .Many significant results had been obtained. The expressions of OPG and RANKL in bone marrow increased in the groups of 4 months with low calcium diet as well as fluoride concentration of 100mg/L or 8 months with low calcium diet,fluoride concentrations of 50 and 100mg/L. Only in the group of 8 months with low calcium diet and...