The Study Of Signal Transduction Mechanism Of Histamine H₃ Receptor On AtT-20 Cell

In the present study , we cultured the AtT-20 cells line from a murine anterior pituitary gland tumor that were reported containing high affinity histamine H₃ receptors and observed the effects of histamine agonists and antagonists on the release of adrenocorticotropic hormone (ACTH). Therefore we investigated the signal transduction mechanisms of histamine H₃ receptors. The contents of development and innovation presented in our study involves the follwing aspects as:1. Detecting ACTH levels in the supernatants of AtT-20 cells by radio immunoassay at the given time points following administrating reagents, we observed that histamine (HA) 10^-6mol/L increased the release of ACTH in time-dependent form and the H₃ receptor-specific agonist R-( α )-methylhistamine (R-(α)-MeHA) 10^-7mol/L mimicked the effects of HA, while the H₁ receptor...