Studies On The Signal Transduction Mechanism Of Laryngeal Squamous Carcinoma Cell Proliferation Induced By IGF

IGF-1 (Insulin-like growth factor 1) and its receptor-mediated signal transduction have evoked wide attention and become one of the hot fields in tumor research because they play an important role in the beginning and development of tumors. In this study, Immunohistochemistry and in situ hybridization technology were first adopted to investigate the expression of IGF-1 and mRNA, IGF-IR, PKC and ERK in laryngeal carcinoma tissue, and analyses were made on the relationship between laryngeal carcinoma cell proliferation, differentiation and metastases. In the meantime, IGF-1 was used to deal with Hep-2 cells of cultured human laryngeal carcinoma, and immunofluorescence, Western blot, Northern blot, PCR-SSCP, LM-PCR, antisense oligodeoxynucleotide and Atlas cDNA expression array were used to observe the effect of IGF-1 on Hep-2 cell proliferation and apoptosis and the relationship between PKC and MAPK activation and cell proliferation. And the functional gene of IGF-1 induced laryngeal carcinoma cell proliferation was analyzed; the involved signal transduction passway and gene regulation of laryngeal carcinoma cell proliferation were explored. This study might lay a foundation for further study on the molecular mechanism of the development of laryngeal carcinoma.1.The distribution and role of IGF-1 and its receptors,PKC,ERK in laryngealcarcinomaAmong 69 specimens, the positive ratio of IGF-1 immunoreaction was 43.48%, and the expression ratio of IGF-1 mRNA was 52.17%, and positive IGF-IR was 46.38%, and IGF-1 mRNA expression was observed in specimens of positive IGF-1 and its receptors. 8 were found metastases out of 10 specimens of high expression of both IGF-IR and IGF-1 mRNA. PKC and ERK...