| Bleeding and perforation in gastrointestinal tract during cardiopulmonaty bypass (CPB) may result in fatal complication. The death rate is high about 67%—72%. Up to now , we have not understand clearly about the changes of the blood flow, oxygen utilization ,kinds of cell factors and hormones in gastrointestinal tract during CPB. We have studied the possible mechanism of gastrointestinal tract injury and assessed more perfect protective method using a rabbit model of CPB . The study includes four parts:PART I Experimental study on changes of gastrin and its receptor and pH of gastric juice with injury of gastrointestinal tract during CPBMethods:In present study, white adult rabbits (n=24) were used. They were divided into two groups: CPB and sham operation group (n=12).In CPB group, CPB were set up after connecting tubes and priming.At the same time, We operated gastric fistula to drainage gastric juice. When the temperature was between 28°C and 32°C, aorta was cross-clamped. The heart arrested with cold St.Thomas II solution (4°C). After 60min,aorta blood flow were reestablished. Part CPB continued to 90min. At 0min,30min,60min,90min,120min and 180min after CPB, we collected gastric juice, plasma. After 180min,pieces of gastrointestinal mucosal were collected and observed with light microscope and transmit electron microscope(TEM).The content of gastrin in plasma or in gastric juice and gastric juice pH were measured. At 180min after CPB, the Wet/Dry ratios, RBC number in gastric juice and gastrointestinal mocusal index were calculated, then we detected the expression of gastrin receptor mRNA in...
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