The Phenomenon And Mechanism Of Insulin Resistance In Cardiomyocytes During Post-simulated Ischemic Reperfusion

Our previous animal experiments showed that accompanied with peripherial insulin resistance, acute myocardial insulin resistance developed after ischemia reperfusion during cardiac operations with cardiopulmonary bypass (CPB), and the magnitude of myocardial insulin resistance was closely related to the heart dysfunction occurrence. Because the elevated anti-insulin hormones and cytokines during CPB as well as the heart functional change can affect the post-ischemic myocardial glucose uptake, so it is necessary to use another model to evaluate whether only ischemia-reperfusion (I/R) can induce acute myocardial insulin resistance.Objective: To accurately evaluate myocardial insulin sensitivity in adult rat cardiomyocytes during post-simulated ischemic reperfusion and the time course, as well as to preliminarily explore the possible mechanism.Methods: 1. An experimental model of simulated ischemia (60min) reperfusion (SI/R) was developed using isolated adult rat cardiomyocytes. 2. The glucose uptake of cardiomyoctyes stimulated by insulin of different concentrations (0iu/L, 0.01iu/L, 20iu/L) was detected with isotopic tracer technique. 3. Insulin-induced glucose transporter 4 (GLUT4) translocation...