The Antitumor Effect Of Beta Defensin 2

BACKGROUD & OBJECTIVE: Murine Beta defensin 2 (MBD2) is a small antimicrobial peptide of the innate immune system. It provided a critical link between the innate immune system and the adaptive immune response. We developed a set of MBD2 gene therapy system and MBD2's inhibition effect on tumor growth , malignant ascite accumulation and the relation with immune system were studied.METHODS: The fragment of mouse beta defensin 2 was expanded from the kidney of BALB/c mouse. MBD2 plasmid was constructed and Lentivirus was produced in 293FT cell. Mesenchymal stem cell (MSC) and CT26 colon tumor were then infected by MBD2-Lentivirus and stably transduced cells were selected. The biological function of MBD2 was evaluated by Dendritic cell migration. BALB/c mice bearing i.p. and sc MethA tumor were treated with MBD2-MSC. The inhibition effect of MBD2 on CT26 tumor growth were tested by tumorigenesis experiment. On the other hand, Mature beta defensin 2 and extracelluar segment of Vascular endothelial Cadherin (VE-Cadherin) were PCR exemplified. For fusion plasmid, (GGGS)3 was used as linker peptide. All the three segments were inserted into pSecTag2B plasmid and transfected CT26 tumor cells.