RNAi Silencing The Hypoxia Inducible Factor (HIF-1α) In Cervical Cancer Cell

Background and Objectives The high-speed growth of the solid tumor will cause relative insufficiency of internal blood supply and decreased oxygen tension. When the tumor physical volume is greater than 1 mm3, its blood supply would not be able to satisfy the demand of tumor cell growth, and some anoxia district will subsequently appear. The decreased oxygen pressure will activate the suppressed hydroxy group of HIF-1α, and HIF-1α will further interact with the enhancer of HRE, which leads to complex stress reactions to hypoxia, inducing tumor neovascularization and increasing glycolysis. The research of Zhong et al found that HIF-1α is expressed in most malignant tumors, but not in benign tumor and normal tissue. Currently there is only very few studies reported the relationship between HIF-1α and growth and development of the gynecologic malignant tumors. In order to establish a research basis for the gynecologic tumor gene therapy, which is targeted at the tumor hypoxia tolerance mechanism, this project measured the HIF-1α expression in multiple gynecologic malignant tumors, and assessed its relationship with hypoxic time and hypoxic severity. After transfecting cervical cancer HeLa cell with the small hairpin RNA (shRNA), which was specifically for HIF-1α, the silencing of HIF-1α gene expression and vascular endothelial growth factor (VEGF) were measured, and whether this activated the apoptosis of tumor cell was assessed. This study will help to identify HIF-1α as a new target for gynecologic tumor gene therapy.Methods 1. The HIF-1α expression in various gynecologic malignant tumor cells was measured by Western blot after being cultured under normal oxygen concentration or in hypoxia condition for 24 hours. The expression of HIF-1α in HeLa cell at different time and under different degree of hypoxia was...