Effect Of Fractalkine On Hypoxic Pulmonary Hypertension

Objective To evaluate the role of fractalkine (FKN) in the pathogenesis of hypoxic pulmonary hypertension, we observed the change of serum and lung tissue FKN level of rats at different phases of hypoxia-induced pulmonary hypertension, investigated the role of hypoxia in synthesis and secretion of FKN in cultured rat pulmonary artery smooth muscle cells (PASMCs) and pulmonary microvascular endothelial cells (PMVECs), and studied the effect of FKN in cell proliferation of cultured rat PASMCs.Methods (1) Thirty-six male SD rats were randomly divided into one control group and 5 hypoxic groups(1d, 3d, 7d, 14d, 21d). Mean pulmonary arterial pressure (mPAP) was measured by a right cardiac catheterization. The right ventricle (RV) and left ventricle plus septum (LV+S) of rat were weighed respectively, and the RV/(LV+S) was calculated. The index of wall thickness of pulmonary arterioles which are the percentage of vascular wall thickness/vascular external diameter (WT%) and the percentage of vascular wall area/total vascular area (WA%) was measured by a computerized image analyzer. The serum and lung tissue FKN concentrations were measured by enzyme-linked immunosorbent assay. FKN mRNA and protein expression in...