The Effects Of Increased Endostatin Expression On Arterial Injury In Rats And Acute Myocardial Infarction In Men

Part One Increased endostatin expression and release are induced by inflammation or hypoxia and both counteracted by atorvastatinObjectives: To study the effect of atorvastatin on the proliferation-inhibiting and proapoptotic roles of endostatin in endothelial cells and also investigate endostatin expression and release induced by inflammation or hypoxia and the effects of atorvastatin on them.Methods: The proliferation and apoptosis of cultured human umbilical vein endothelial cells (HUVECs) were tested by the MTT assay and by ethidium bromide and acridine orange staining, respectively. The concentrations of endostatin in cell culture supernatants were measured by ELISA. Meanwhile, endostatin mRNA in cultured cells was detected by RT-PCR.Results: The MTT assay and the ethidium bromide and acridine orange staining revealed that endostatin dose dependently inhibited the proliferation of cultured HUVECs and induced the apoptosis of cultured HUVECs, respectively, which were attenuated by atorvastatin dose dependently. Compared with control, ELISA assay showed that the concentrations of endostatin in supernatants elevated significantly after...