| In last decades, with the development of techniques of laser and signal detection, the photoacoustic method has been applied in various research fields, such as chemistry, physics, medicine and biology. The Laser-induced Time-resolved Photoacoustic Calorimetry (PAC) has received recognition as an effective experimental method for a direct to measure both thermodynamics and kinetic properties of photo-induced reactive intermediates. Based on the strong temperature dependence of the thermal expansion coefficient in aqueous solution, it is now feasible to measure the dynamics of both volume and enthalpy changes accompanied with the photoinduced ligand dissociation on the time scale of nanosecond to microsecond. In this work, we study the photolysis of coenzyme B12 analog 2′, 5′-dideoxyadenosylcobalamin and oxyhemoglobin using photoacoustic calorimetry to obtain energetic information of photoreaction dynamic and to give evidence of biological function and enzymatic reaction mechanism.It is well known that nanotechnology is a powerful platform for 21st century science and technologies. The materials exhibit unique and very valuable properties when it is"nanosized". In view of the fact that scale of nanoparticle is smaller than the size of cell, combination of the nanotechnology with biology and medical science had attracts more and more interests on the world. It is applied to many aspect, such as, cell isolation, cell dye, specific medicine or new type of antibody on medical therapy. In this dissertation, we successfully self-assemble a series of protein molecules on the surface of silicon substrates, and synthesize a kinds of nano-magnetic particles. Subsequently, the composition, structure and morphology of new materials were determined, and their biological application were studied.
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