Experimental Studies On Separating, Culturing And Differentiating Of Adipose Tissue Derived Stem Cells From Rats And The Cellular Compatibility With Silk Fibron

Tissue engineering is a multidisciplinary area of research aimed at the regeneration of tissues and the restoration of the function of organs by the implantation of cells or tissues grown outside the body, or by the stimulation of cells to grow into an implanted matrix. The general pri nciple of tissue engeering involves the combination of living cells with a natural or synthetic support, or scaffold to produce a three-dimensional living tissue construct which is functionally, structurally and mechanically equal to, if not better than, that which it has been designed to replace. Seeding cells are the key point in tissue engeering. Several groups have demonstrated the differentiation of mesenchymal stem cells obtained from human lipoaspirates. The purpose of this study was to examine whether the adipose tissue from rats contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages and the compatibility with the silk fibron.PARTâ… :The study on separating, culturing and facial type of adipose tissue derived stem cellsObjective To explore the methods of culturing and identify the adipose tissue derived stem cells in vitro in order to offer the ideal seeding cells for bone tissue engeering.Methods First, rat epididymal fat pads were isolated and processed from Sprague-Dawley rats into a fibroblast like cell population; next, to culture the cells in vitro. Moreover, the cells were observed by transmission electron microscope and studied the CD44, Vimentin antigen by indirect immunofluorescence methods.Results The highly pured adipose tissue derived stem cells were cultured in vitro and...