Impact Of Growth Hormone On Cell Cycle Kinetics Of Pancreatic Cancer Cells And Significance Of Growth Hormone Receptor-JAK2-STATs In Signaling

Growth hormone has been shown to accrue lean mass tissue and improve nitrogen balance in clinical and experimental catabolic states. In addition, it has been shown that growth hormone can preserve the nutritional status of the tumor-bearing host. Previous research has demonstrated a selective anabolic effect of growth hormone on host muscle protein in animals and on tumor implants. As a matter of fact, the tumor-bearing state is associated with abnormalities in carbohydrate, protein, and lipid metabolism. Growth hormone has been widely applied in GH-deficiency patients, and it has been confirmed that GH can promote immune functions . Malnutrition and negative nitrogen balance greatly discounts s curative effect and rise fatality rate, so it's urgent to ameliorate the whole nitrogen blance; Nevertheless, concern of possible mitogenic influences of GH such as those observed in lymphoid malignancies could preclude the use of GH in cancer-bearing hosts.The ideal goal of the therapy with growth hormone is to selectively promote host anabolism without stimulating tumor growth. This experiment was designed to try to illustrate the effect of GH on proliferation pancreatic cancer cell and the possible molecular mechanism. Relevant experimental protocol and results were provided as follows:Part A Impact of GH on proliferation of pancreatic cancer cells and application value in tumor-bearing hostsCells during exponential growth stage were harvested and cultured in medium containing growth hormone of various concentrations (50ng/ml, 100ng/ml); After 24, 48,72 hours, cells were counted using a Coulter Counter. Cells cultured for 24 hours were made into single cell suspentions and samples underwent subsequent cell cycle kinetic evaluation. The results revealed GH stimulated cell growth in vitro; Growth hormone also elevated percent S phase (50ng/ml: 52.00±14.83vs30.15±6.22, and 100ng/ml: 53.21±15.49vs30.15±6.22, respectively, P<0.05) and proliferation index (50ng/ml: 0.72±0.09vs0.52±0.13, and 100ng/ml: 0.70±0.09vs0.52±0.13, respectively, P<0.05) of cells after 24-hour culture.