Radionuclide Imaging And Antitumor Effect Mediated By Coexpression Of Somatostatin Receptor Type 2 And Cytosine Deaminase On Nude Mice Xenografts

The studies of reporter gene imaging, radionuclide on diagnosis and therapy of tumor in gene therapy using suicide genes are of great importance in the study on diagnosis and therapy of tumor. Combined the three methods will be of great benefit to the diagnosis and therapy of tumor, and there are little reports published about this in internal journal. In our study, we have used somatostatin receptor type 2 (SSTR2) as reporter gene, cytosine deaminase (CD) as suicide gene and followed by construction of the bicistronic expression vector of CD and sstr2. Than the vector was further transfected in vitro to human lung adenocarcinoma A549 cells which were then selected with G418. And acquired a positive cell clone called it pCIS-A549. The selected positive clone cells were...