Construction Of Human Combinatorial Immunoglobulin Library On The Surface Of Phage And Selection Of HAV Antigen-Specific Monoclonal Antibodies

Over the last century animal antiserum, and more recently rodent monoclonal antibodies, have been used clinically to neutraliza toxins, and to treat bacterial and viral infection. However, the use of animal antibody can lead to an antiglobulin response and hypersensitivity reactions. Ideally human monoclonal antibodies would be used, but it is difficult to make them. Not only are peripheral blood lymphocytes a poor source of the blast cells that are actively involved in the immune response, but it is difficult to immortalize them. Instead of immortalizing B—cells for produc-...