The Express And Regulation Of GDNF MRNA In Facial Nerve-striking-model And The Promotional Effect Of GDNF To Nerve Regeneration

With the rapid development of traffic vehicle, the incidence of traffic accident is going up. Because of the position, the facial nerve is easily to be injured. Though there are many ways to cure the injured nerve, the course of nerve recovery need a long time and the effect is not confirm, so, we have to go on our works in order to find out more efficiency methods. Nowdays, many kinds of neurotrophic factors (NTFs) which have biological effects to the motoneurons are cloned, they have been approved to have trophic and protective functions to motoneurons. These functions relate to the growth, differentiation, death, repair and regeneration of motoneurons. Of all the NTFs, the Glial line-cell derived neurotrophic factor (GDNF) has the most prominent effect. At the beginning, GDNF was expected to cure the ALS, and Parkinson's desease, but it's powerful trophic and protective functions to the motoneurons makes it have potential application value to the regeneration after motoneurons is injured. In this experiment, we first established a striking model in rabbits, then we discuss the express and regulation mechanism of GDNF mRNA in injured motonurons and axons, analyse what role does GDNF played after motor nerve is injured. Finally we use external GDNF to the injured facial nerve to see whether GDNF can enhance the regeneration of injured facial nerve. The experiment shows that:With 10m/s velocities,7.5J striking energy can make Ⅲ—Ⅳ° injury to the rabbit's facial nerves, the structure of the nerves are destroyed, but the membranes of the nerve are completed, so it can be regarded as a steady facial-nerve striking model. Increase the striking velocity, rabbits...