Human OP-1 Gene Cloning, Preparation Of Polyclonal Anti-sera And Expression In Mammalian Cells

Osteogenic protein-1 (OP-1) or Bone morphogenetic protein-7(BMP-7), originally founded by Ozkaynak is a member of transforming growth factor-beta superfamily. Human OP-1 cDNA was cloned by means of digonucleotide probes based on the bovine OP peptide sequences and their relationship with Dpp/Vg1 subgroup. Full length of the cDNA is 1878 bp, open reading frame of 1296bp encodes 431 amino acid residues. Mature protein of BMP-7 is glycoprotein which is composed of disulfide-linked dimers. Like other BMP members OP-1 can induce bone formation, restore bone and osteochondral defects, promote bone remodeling etc. So OP-1 may have many therapeutic uses in dentistry, orthopaedics and so on. Since the sources of native OP-1 are limited, so it's necessary to develop ways to produce OP-1 in vitro. The development of gene engineering made it possible. The following studys are based on the considerations above. 1. Mature peptide gene cloning of OP-1 and preparation of polyclonalanti-sera.