Cloning And Expression Of Genes In Dorsal Root Ganglia Of The Rat After Peripheral Axotomy

After peripheral axotomy, the expression of many genes has been changed in dorsal root ganglion neurons. In previous studies, the regulation of the expression of the genes cloned from normal tissue has been studied with in situ hybridization and immunohistochemistry. The expression of some genes is up-regulated, for instance, galanin, neuropeptide Y, neuropeptide Y receptors and nitric oxide synthase. However, those results are still not sufficient for the understanding the molecular and cellular mechanisms of the disease occured following peripheral axotomy, especially chronic pain. In present study, we used cloning techniques to clone the differentially expressed genes in dorsal root ganglia (DRGs) of the rat after peripheral axotomy.