Study On The Effect Of Hepatitis B Virus-encode X Protein On Apoptosis Of Hepatocellular Carcinoma Cells And Its Relationship With P53 And Nuclear Factor Kappa B

Hepatitis B virus (HBV) and hepatocellular carcinoma is closely related, and HBV associated hepatocellular carcinoma is one of the most frequently encountered cancers. The product of HBV x gene has transactivation function and transactivate many viral and cellular genes. X protein has the ability of malignant transformation on hepatocytes. X protein also plays an important role in carcinogenesis of hepatocellular carcinoma. This study investigated the effect of the X protein of hepatitis B virus on apoptosis of hepatocellular carcinoma cells and its relationship with p53 and nuclear factor kappa B. The main methods and results were as follows:1. To investigate the effect of the X protein on the maglinant phenotype and apopotosis of hepatocellular carcinoma cells, HBV x gene was firstly obtained from the plasmid pTTHK using polymerase chain reaction (PCR). The PCR product was ligated to the plasmid pT7Blue for sequencing. Then, HBV x gene was inserted into the vector pCDNA3.1 to construct the eukaryotic expression vector pCDNA3.1-HBX. Gene transfection mediated by the lipofectamine was used to introduce HBV x gene into human hepatocellular carcinoma cell line HCC-9204. The cell clones...