A Study On The Interleukin 2-Pseudomonas Exotoxin Fusion Proteins

It is well known that activated T, B or monocytes express high affinity IL-2 receptors, whereas normal resting T, B or monocytes and their precursors do not.In contrast a proportion of abnormal cells in certain lymphomas or leukemias, some autoimmune diseases and organ allograft rejection express high level IL-2Rs with high affinity, and these abnormal cells play the pivotal roles in the development of the above -mentioned diseases.Moreover, HIV-1 can replicate only in the T cells of the activated state after the viruses infect CD4⁺ T cells, otherwise in resting T cells the viruses exist in a latent state.Psudomonas exotoxin (PE), a 613 amino- acid single chain protein with 66KDa molecular weight, is composed of three different domains: Domain I a (1-252aa) for cell recognition; Domain II (253-364aa) for translocation of the toxin across a cell membrane, and Domain III (400-613aa) for ADP-ribosylation of elongation factor 2. PE40 and PE66^4Glu are PE-derived mutants of deleted cell-recognizing domain or...