A Study On The Molecular Mechanism Of Nm23-H1 Gene For Reversing Invasive And Metastatic Phenotype Of Lung Cancer Using Site-directed Mutagenesis

Background and objective Invasion and metastasis is not only the malignant phenotypes of lung cancer, but also the main cause related to cancer death. It has been proved that invasion and metastasis in lung cancer is a complicate course with multiple step and multiple stage;and regulated both by metastasis suppressor genes and metastasis promoting genes.Therefore, it is very important to study and elucidate the molecular mechanism of invasion and metastasis in lung cancer. Nm23-H1 was originally identified as a tumor metastasis suppressor gene; its functional and structural abnormality was closely correlated with the invasion and metastasis of cancer. Our previous research results have proven that the reduced expression and hetero-deletion of nm23-H1 gene was closely correlated with the high metastasis potential and poor prognosis of lung cancer. Transfection of the wild type nm23-H1 gene can reverse the...