Establishment Of Multidrug Resistant Cell Line (HepG2/mrp1) Transfected The Mrp1 CDNA Into HepG2 And The Study Of Reversing MRP1 With Hepatocellular Carcinoma By RNA Interference

BACKGROUND AND OBJECTIVE: Liver cancer is one of the most common cancers and the third leading cause of digestive system tumor related mortality in Chinese. There were 110,000 deathes each year and is about 45% of the worldwide deathes with liver cancer. The important cause are poor efficiency of treatment and highly recurrence. Chemotherapy remains the major approach of liver cancer therapy. One of the major problems in chemotherapy is multidrug resistsnce (MDR) against anticancer drugs. Multidrug resistance-associated protein (MRP1) is a ATP-binding cassette transporters that mediate MDR. In the present study, the human full-length mrp1 cDNA were transfected into human hepatocellular carcinoma cell lines (HepG2) and MDR obtained because of overexpression of MRP1. Then, RNA interference was performed to reverse the MDR of the multidrug resistant cells.