Co-culture System Of MSCs And Aβ_1-40 Injured PC12 In Vitro

PrefaceIt is common knowledge that AD (Alzheimer' s disease, AD) is an untreat-able degenerative disease with very few temporary and palliative drug therapies. MSCs grafts present a potential and innovative strategy for the treatment of AD, with the possibility of providing a more permanent remedy than present drug treatments. Aβ play an significant role in the apoptosis of neuron, exorbitant ap-optosis of cells may be one of the important reasons that caused gradually dysfunction in nervous system of Alzherimer's disease; some of amyloidf} -peptide fragment (include Aβ_1-42,Aβ_1-42,Aβ_25-35 could injured cultured cells in vitro mainly via apoptotic pathway , Most of AD cell models adopt PC12 cells. The present article aims to establish the co - culture system of MSCs ( Marrow Stro-mal Cells, MSCs)and Aβ_1-42 injured PC12 in vitro , PC12 was transferred by tr-answell; Investigate the effect and mechanisms of the system to improve the differentiation into neural cells of MSCs and inhibit apoptosis of PC12 induced by Aβ_1-42 To investigate the role of Phosphotate - STAT3 in MSCs of the co - culture system . So grafted MSCs have the possibility to inhibit apoptosis of neuron induced by Aβ in the diseased brain to promote recovery.Method1. To culture and observe of MSCs2. To examine MSCs by immunofluorescence stain : immunofluorescence stain was performed to examine the species of MSCs. First antibody was used mesenchymal marker CD44, Fluorescent secondary antibody was used FITC.