Analysis Of Genes Expression Of The Human Umbilical Vein Endothelia Cells

IntroductionEndothelial cells (ECs) are very important for many diseases, for example, coronary artery disease, and tumor. Coronary artery disease is result of ischemia and hypoxia of myocardial cells and endothelial cells of coronary artery. Stress, inflammatory reaction and lipidoese of ECs are the major mechanism of coronary artery disease. Another, angiogenesis take part in tumorigenesis, invasion and metabasis. In short, ECs is very important for many diseases. As we known, genes is same important in the process of diseases. What will induce the difference expression of genes of ECs in the process of diseases? What is its effect? To date, there is not overall analysis about gene expression of ECs. In the same time, this is significant for gene therapy.Object:To analysis all the gene expression profile of ECs through GeneChip of Affymetrix and get the important signals pathway, key genes and molecular function.Methods:(1)Gene expression profile of human umbilical vascular endothelial cells (HUVEC) is analyzed by the Affymetrix GeneChip HG-U133A plus2.0 and confirms the results of GeneChip with Real-time PCR.(2)To mine the data by the bioinformatics software of internet. For example, Microsoft Excel, Milano, Go, PANTHER, Genomatix etal.Results:...