Generation Of The Recombinant Adenovirus Expressing HBV X-HCV C Fusion Gene And Its Biologic Impact On Human Hepatocytes

Hepatocellular carcinoma (HCC) is the second most frequent cancer in China. Many etiological factors have been related with HCC development, chronic infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) and prolonged dietary exposure to aflatoxin are responsible for about 80% of all HCC in human. Chronic infection with HBV or HCV often results in cirrhosis and enhances the probability of developing HCC. The underlying mechanisms that lead to malignant transformation of infected cells,however, remain unclear. Based on epidemical data, superinfection with HBV and HCV is concerned to the increased frequency of developing HCC, but the relative mechanism remains unclear. It has been reported that HBV X gene and HCV C gene both play important role in hepatocarcinogenesis. The fact that HBV X gene and HCV C gene induce HCC in transgenic mice offered more evidence for the relationship between these genes and HCC.Disbalance between proliferation and apoptosis of hepatocytes maybe contributes to the pathological processes of hepatocarcinogenesis. Both HBV X protein and HCV C protein are multiple-functional protein, they can deregulate cell cycle check points, transactivate or activate cellular and viral genes, which involve in transcription regulation, signal transduction pathway, cell cycle regulation, etc. so they may deregulate the cell cycle and apoptosis of the hepatocytes and maybe have the common target point, but it was still unclear that if there was synergism of HBV X protein and HCV C protein on hepatocarcinogenesis.