The Visible Screening Of Dnazymes And Their Inhibition Of Latent Membrane Protein-1 Gene Expression In Nasopharyngeal Carcinoma

Nasopharyngeal carcinoma (NPC) is a common human malignancy in China. Latent membrane protein-1(LMP1) of Epstein-Barr virus (EBV) plays a significant role in the pathogenesis of NPC and is regarded as the oncogene of NPC. DNAzyme(DZ) is a novel molecular biological tool which could cut RNA substrate between an unpaired purine and a paired pyrimidine residue to regulate the expression of target genes. Our research aimed at 1) synthesizing series of 10-23 DNAzymes(10-23DRz) against the LMP1 mRNA 2)investigating the cleavage variability of DNAzymes in vitro 3)detecting the inhibitive effect of LMP1 gene expression by special DNAzyme 4)establishing a visible system to estimate the cleavage ability of DNAzymes and 5)starting a novel therapeutic method in NPC.Objective:1. To investigate the existence of EBV-LMP-1 gene from plasma in both NPC and non-NPC patients in Chongqing province, western China and the loss of an Xho I-site within N-terminal region and a 30bp deletion in...