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Preliminary Study On Chromosomal Integration Of Epstein-Barr Virus In A Burkitt's Lymphoma Cell Line (Raji)

Posted on:2007-01-14Degree:DoctorType:Dissertation
Country:ChinaCandidate:J M GaoFull Text:PDF
GTID:1104360185486680Subject:Pathology and pathophysiology
Abstract/Summary:PDF Full Text Request
Discovered in cultured lymphoblasts from Burkitt's lymphoma (BL) in 1964 by Epstein and Barr, Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several malignancies, primarily of lymphoid and epithelial cell origin, including Burkitt's lymphoma and nasopharyngeal carcinoma (NPC). EBV DNA is detected as a linear form in cells at the lytic phase of EBV infection, but during latent infection the viral genome is maintained in episomal form through the fusion of genomic termini. Most BL and NPC tumors harbor the episomal EBV genome, representing persistent latent infection. Chromosomal integration of EBV DNA provides an alternative way of establishing persistent infection and may represent another mechanism of virus-cell interaction. Integration of EBV into cellular genome may enhance chromosomal instability, and alter the functions of the genes with critical roles in cell proliferation, apoptosis and differentiation.The Raji cell line was established in 1963 from a Burkitt's...
Keywords/Search Tags:EBV, Raji, NPC biopsy tissue, integration, genomic library, FISH, oncogenic mechanism
PDF Full Text Request
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