The Protective Effect Of Hypercapnic Acidosis Preconditioning On The Heart And Blood Vessel

Part â… Protective Effect of Hypercapnic Acidosis Preconditioning on Rabbit Heart Suffered from Ischemia-Reperfusion injuryObjectiv This study observed the protective effect of hypercapnic acidosis preconditioning on rabbit heart suffered from ischemia-reperfusion injury.Methods The animals achieved hypercapnic acidosis with mechanical hypoventilation before ischemia-reperfusion.32rabbits were randomly divided into four groups(n=8) according to the acidification degree: hypercapnic acidosis group A (group A), hypercapnic acidosis group B (group B), hypercapnic acidosis group C (group C), ischemia and reperfusion group(group IR). Animals in group IR were ventilated normally:tidal volume 15ml/kg,breathing rate 35 bpm .The PETco2 was matained at the level of 40～50 mmhg for 30 minutes. Animals in group A,B,C received low-frequency ,low-volume ventilation to achieve hypercarbonic acidosis ,The target value of PETCO₂were75～85,65～75, 55～65 mmhg respectively.This value was maintained for 5 minutes.The animals then were ventilated normally to make the PETCO₂ return to 40～50 mmhg . Animals in each group received ligation of left anterior branch artery for 30 minutes and reperfusion for 180 minutes. infarction size was calculated. Morphology of cardiomyocytes were observed.The ECG and hameodynamic were monitored continuously, Acid-basis balance was measured during procedure...