Study The Effect On Expression Profiling Of Hepatocelluar Carcinoma (HCC) By Trichostatin A, A HDAC Inhibitor

Hepatocelluar carcinoma (HCC) is a major type of primary liver cancer and one of the most frequent human malignant neoplasms. Common risk factors of human HCC include chronic hepatitis virus(HBV and HCV) infection, dietary aflatoxin Bl(AFBl)ingestion, chronic alcohol abuse and cirrhosis associated with genetic liver diseases. Hepatocarcinogenesis is the result of interaction between hereditary and environmental factors. Hereditary factors determine individual susceptibility to cancer and environmental factors determine which one will suffer from cancer. Studies of genetic and epigenetic mechanisms of hepato-carcinogenesis showed that HCC development is a complex polygene and multi-pathway process. The core biological basis of hepato-carcinogenesis is the activation of proto-oncogenes and the inactivation of tumor suppressor genes induced by genetic and epigenetic alterations. These abnormal expressed gene include: p53, Rb, ras, c-myc , u-PA (urokinase type plasminogm) , MDM2 (murine double minute2),MAGE (melanoma antigen), MMP(matrix metalloproteinase), p21WAFl/CIP1, P27kip, PTEN, E-cadherin, and so on.Alterations in chromatin structure result from histone...