Effect Of Transforming Growth Factor Beta 1 Gene Transfection Combined With FTY720 Administration On Rat Cardiac Allografts

Part One The Model of Rat Heterotopic Heart Transplantation With Modification of Heron's TechniqueObjective The goal of this study was to establish a simplified and authentic cervical model of rat heterotopic heart transplantation by modified Heron's technique with cuff vessel anastomosis. Methods We chose the Sprague-Dawley rat as the recipient and the Wistar rat as the donor. The main procedure was as follow: After anaesthesia the donor heart was procured. Then the recipient was heparinized. The bole of the right external jugular vein was sheared at the site of more branches and the proximal of it was pulled by 7/0 polypropylene suture through a self-made canula. The wall of the vein was sheared vertically and evaginated to encase the wall of the canula, fixed by a silk suture. The right common carotid artery was prepared in the same way. Then the donor heart was put in the operating field, the canula of common carotid artery was put into the donor's ascending aorta and fixed outside, then the canula of external jugular vein was put into donor's pulmonary artery and also fixed. Results All the operations were performed by a single surgeon with negligible operative risk. There were no anastomosis leakage and vessel obstruction after recirculation. Conclusions This model of the heterotopic heart transplantation is a simple, economical, reliable, practicable and high reproducible model and it provides a satisfactory platform for our following studies.