Experimental Studies On The Key Biological Technologies To Emergency Treatment For Acute Radiation Injuries

In this article, four parts are included in the experimental studies on the key biological technologies to medical emergency treatment for acute radiation injuries. The molecular biological mechanisms of acute radiation injuries and the molecular biomarkers of assessing radiation dose are expatiated. The briefly summarization is as follows.Part 1 Experimental study on characteristic of radiobiology to umbilical cord blood AC133+ cellsObjective To investigate the characteristic of radiobiology to umbilical cord blood AC133+ cells including the features in relation to the separation and purification of normal and irradiated umbilical cord blood AC133+ cells, the effects of different cytokine combinations on the expansion efficiency ex vivo and their influence on the migration and homing capabilities of normal umbilical cord blood AC133+ cells and the effects of cytokine combinations on the cell cycle and early apoptosis of irradiated umbilical cord blood AC133+ cells so to provide the experimental basis for its clinical application. Methods The umbilical cord blood AC133+ cells were separated and purified by magnetic-activated cell separation (MACS) purification system and the purity of AC133+ selected cells were estimated by flow cytometry. The expansion capacity of the umbilical cord blood AC133+ cells, which were freshly isolated and incubated in serum-free liquid culture system for 7 d and 14 d with different cytokine combinations ex vivo, was tested by flow cytometric immunofluorescence methods. The CXCR4, CD49 d, CD11a and CD62L expression on the surface of umbilical cord blood AC133+ cells which were freshly isolated and incubated in serum-free liquid culture system for 7 d and 14 d with different...