Comparative Proteomic Analysis Of Endometriosis

BackgroundEndometriosis is a common Gynecological disease affecting women during their reproductive age. It is defined as functional endometrium appearing in place other than the uterus causing dysmenorrhoea and infertility. The focus of endometriosis is widely-spread aggressive and easily recurrent. Up to now clinical diagnosis and treatment is far from satisfactory. Basic research shows eutopic endometrium is different in adhesion aggression and angiogenesis from normal endometrium, thus plays an important role in pathogenesis of endometriosis. Onset of endometriosis is determined by the characteristics of eutopic endometrium, which refluxes into the abdominal cavity during menorrhea. With the progress in Human Genome Project and finish of Genome nucleotide acid sequencing, mankind entered post-Genome era. Proteomics becomes the frontier of life science. The research of disease-related genes and its function becomes the key of this work. Using proteomics technique we can separate and identify differently expressed proteins in eutopic endometrium adenomyotic tissue and body fluid of the patients with endometriosis, which helps us not only clarify the pathogenesis but also plays an important role in early detection treatment choice follow-up and screening new drugs.ObjectiveTo set up the protein expression profiling of eutopic endometrium adenomyotic foci and plasma of patients with endometriosis, identify the different expressed proteins and investigate the regularity of protein expression during pathogenesis. To clarify the mechanism of endometriosis in proteomics and provide new target for clinical diagnosis and treatment. MethodPart â…  Using two-dimensional electrophoresis to setup protein expression profiling of eutopic endometrium in patients with endometriosis and normal endometrium and identify the differently expressed proteins.Part â…¡ Using two-dimensional electrophoresis to setup protein expression profiling of adenomyotic foci and normal uterine muscle and identify the differently expressed proteins.