Human Hemangiopoietin Promotes The Proliferation Adherence And Chemotaxis Of Human Bone Marrow Hematopoietic Stem CD133～+ Cells

purpose To investigate the effect of human hemangiopoietin (HAPO) on human bone marrow hematopoietic stem CD133~+cells and identify its biological function. Methods Liquid culture, semi-solid culture, fluorescence activating cell sorter (FACS) detection, and microscope counting were carried out. Result 1. After 7 days of liquid culture, there were 14.78% more percentage of CD133~+ cells (56.70%) in HAPO (100ng/ml) group than that in control group (41.92%) according to the result of FACS test. The number of CD133~+ cells increased from 6.8×10~4 to (1.79±0.02)×10~6(about 41.98 times) in HAPO group. The number of CD133~+ cells increased from 6.8×10~4to (2.88±0.12)× 10~6 (about 41.98 times) in HAPO group. The number of CD133~+ cells increased from 6.8×10~4 to (1.79± 0.02) ×10~6(about 26.09 times) in control group. After 10 days, there were 49.02% percentage of CD133~+ cells in HAPO (100ng/ml) group and in control group (32.60%). The increased times of CD133~+ cells in HAPO (100ng/ml) was 356. The increased times of CD133~+ cells in control was 98. 2. After 7 days liquid culture, FACS analysis showed that HAPO (100ng/ml) promoted expression of CD49d (88.17% in HAPO group, 80.11% in control). 3.