Study On The Mechanism Of Endothelial Cell Injury Mediated By Antiendothelial Cell Antibodies And The Indentification Of Endothelial Cell Autoantigens

ObjectiveTo investigate the clinical significant of antiendothelial cell antibodies (AECA) , establish autoantibody-mediated vascular endothelial injury model, evaluate their ability to induce the apoptosis of endothelial cell, and identify the endothelial cell autoantigen in systemic vasculitis by the proteomics technique.Method1. To detect AECA by using methods of Cell-ELISA, indirect immunofluorescence(IIF) and western blotting, and analyze their relationships with the disease activity and clinical manifestation.1) With HUVEC as substrate cell, sera from 129 systemic vasculitis patients (including 59 Behcet' s disease (BD) , 28 Takayasu arteritis (TA), 20 Wegener's granulomatosis (WG), 8 polyarteritis nodosa (PAN), 9 microscopic polyangiitis (MPA), 5 Churg-Strauss syndrome(CSS)), 57 SLE,25 RA and 100 healthy donors were screened for the presence of AECA by using fixed cell-ELISA. The results of AECA by ELISA are also analyzed with their relationships with the disease activity and clinical manifestation.2) With HUVEC as substrate cells, the detection of AECA by using cell-ELISA and IIF from 103 patients with a variety of systemic vasculitis were compared and correlated.3) With HUVEC and EA.hy926(endothelial cell line) as substrate cells, the detection...