Immunotherapy Effects Of Dendritic Cells Plused With Tumor Antigen In Combination With CpG Oligodeoxynucleotide On Bladder Tumor

Bladder tumor is the most common tumor in urinary system of domestic people, which is characterized as high incidence rate and high recurrence rate in clinic. The devolpment and recurrence of bladder tumor is related to the low immunological function of host. How to raise the immunological function of patients with bladder cancer is the key to treat and prevent recurrence of bladder tumor.Dendritic cells are believed to have the most powerful antigen presenting capacity and the native T cells can be only primed by DC. With the intensive understanding to the biological behaviors of DC, people pay more and more attention to the role of DC in cancer immune therapy. Dendritic cell-based vaccine manifests a good clinical applications as a representative of new vaccinesRecent studies have shown that immunostimulatory activity of Bacillus Calmette-Guerin resides in its DNA fraction and that unmethylated CpG motifs in bacterial DNA are responsible for this activity. Synthetic oligodeoxynucleotides(ODNs) containing CpG motifs have the same activity as bacterial DNA. CpG-ODNs are recognized by specific Toll-like receptor 9 on the surface of DCs and induce an activataion phenotype of DCs characterized by the expression of costimulatory molecules, enhanced antigen presentation to T cells and secretion of T helper 1 (Th1)-promoting chemokins and cytokines, which trigger a wide range of secondary effects, such as natural killer(NK) cell and monocyte activation, which have antitumor activity. As a new immune adjuvant, CpG-ODN may be used in clinic because of high effectiveness and safetyFor immunotherapy of tumors, CpG-ODN have been successfully used in combination with tumor-specific antibodies and cytokines as a vaccine adjuvant, but is seldomly combined with DC-based vaccine, especially in baldder tumor. In the present study, we investigated firstly the functional status of tumor infiltrating dendritic cells (TIDC) in bladder cancer. Secondly, the effect of CpG-ODN on activating DCs was evaluated. After...