Intrahippocampus Transplantation Of Neural Stem Cells Modified By Noggin Gene For Aging Mice

During normal brain aging, numerous alterations develop in the physiology, biochemistry and structure of neurons and glia. Aging changes occur in most brain regions and, in the hippocampus, have been linked to declining cognitive performance in both humans and animals. Age-related changes in hippocampal regions also may be harbingers of more severe decrements to come from neurodegenerative disorders such as Alzheimer's disease (AD). Alzheimer dementia is a neurodegenerative disease characterised by loss of memory and other cognitive functions. Neural stem cells (NSCs) graft present a potential and innovative strategy for the treatment of many disorders of the central nervous system including AD, with the possibility of providing a more permanent remedy than present drug treatments. After grafting, these cells have the capacity to migrate to lesioned regions of the brain and differentiate into the necessary type of cells that are lacking in the diseased brain, supplying it with the cell population needed to promote recovery. Stem cell-based therapy to replace lost and/or damaged cells in the aging brain is currently the focus of intense research.Neural stem cells (NSCs) are isolated from the mouse CNS at 1990s. NSCs is characterized by: 1) undifferentiated feature, 2) self-renewing capacity, 3) multipotentiality. In the other words, NSCs can be passaged and cultured for a long time and proliferate in vitro. Induced by some factors, NSCs could differentiate into neuron, astrocyte and oligodendrocyte. The neurons differentiated from NSCs have the whole electronic physiological function and will make synapses and integrate with host brain after intracerebral implantation. So are NSCs the ideal vihicle cells. In the present study, we have obtained efficient recombinant adenovirus transduction of noggin gene into proliferating NSCs in culture, and we report that NSCs engineered in this way can induce functional effects after intrahippocampus transplantation in the aging model mice. The main methods and results are as follows:1.The pAdEasy-1-noggin recombinant adenovirus plasmids and pAdEasy-1-GFP...