Studies On The Tissue And Cell Culture And Ginkgolide B Production Of Ginkgo Biloba L.

The Ginkgolides (GK), A, B, C, J, M and Bilobalide (BB) are a unique family of compounds which are present only in Ginkgo biloba L. Ginkgolides show a significant pharmacological activity against the Platelet Aggregating Factor (PAF), and GKB is the best. These ginkgolides have been proved to be effective treatment for asthma, endotoxin shock, rejecting reaction of organ transplantation, cardiovascular disorder and inflammatory reactions. Up to now, the leaves of Ginkgo trees are still only the resource for ginkgolides, alternative resources, such as chemical synthesis, plant tissue and cell culture, have been investigated. From a long-term point of view, production of ginkgolides by plant tissue and cell culture may be a promising choice. In this thesis, the researches of callus induction, callus culture, cell suspension culture, cell bioreactor culture, as well as single-cell cloning and regenerated roots induction were studied.1. Among 3 single plant growth regulators (NAA, 6-BA, 2,4-D, NAA), NAA was the most effective for callus induction from leaves, petioles and young stems. Expect endosperms, from the other tested explants (embryos, cotyledons, leaves, petioles, radicles) calli could initiate. The callus formation rates from embryos, cotyledons, and petioles could reach 100.0%, the rate from radicles was the lowest, at 33.3%. The rates from leaves and young stems in light were slightly lower than those in the dark, but on the contrary for petioles and radicles. The media MS and DCR were both suitable for callus formation from leaves and petioles.2. 1) The method of extraction, pre-purification and HPLC determination of...