Study On Prognostic Factors Of Lymph Node-Negative Breast Cancer

Objectives: Analyze the relationship between clinical parameters and prognosis of lymph node-negative breast cancer patients. Detect gene amplification and protein expression of HER2, TOP2A, CCND1 and EMS1 in some cases, and try to find out the association of the genetic changes and prognosis.Materials and Methods: Collect the clinical data and survival information of lymph node-negative breast cancer patients in Cancer Hospital of CAMS from 1995 to 1999. Using Kaplen-Meier survival analysis and Log-rank test to evaluate the association of clinical parameters and prognosis. Choose 80 patients into 2 arms with randomized paired design (good-prognosis with DFS<5 years, and bad-prognosis with DFS>5 years). Detect the gene amplification with real-time PCR from DNA samples extracted from formalin-fixed, paraffin-bedded tissues. Examine the protein expression with immunohistochemical methods in tissue array. Evaluate the difference of gene amplification and protein expression in these 2 arms with chi-square test.Results: Median age of all patients is 48 years, 57.5% of the patients are premenopause, tumor size is less then 2cm in one half cases, 58.4% have positive ER status. Half of the patients received adjuvant chemotherapy after surgery. Adjuvant hormonal therapy was given to 54.9% of all patients. 268 cases were disease-free survived till Dec 31^st 2005. There are 57 loco-regional or distant relapse and 16 secondary malignancies. 5 years DFS and OS is 82.7% and 94.1 respectively, estimated 10 years DFS and OS is 72.0% and 81.4% respectively. Patient age, tumor...