The Research Of Damage Mechanism In PC12 Cells Induced By Rotenone

1 Effects of rotenone on mitochondria and ultrastructures in PC12 cellsObjective: Mitochondria is mainly energy of metabolic site in animal ceil. Mitochondrial dysfunctions play an important role in aetiology of Parkinson's disease. Rotenone, a lipidphilic insecticide, is an inhibitor of mitochondrial complex I . In this part of experiment, the effects of rotenone on reactive oxygen species (ROS), mitochondrial transmembrane potential (with expression of (?)) and morphological changes were tested. The aim is to investigate the me(?)hanism of mitochondrial dysfunction induced by rotenone.Methods: Using incubated adreno chromaffmoma (PC 12) cells in mice, divided into 1 control group and 4 experimental groups. When cells developed well, the control group was refreshed with culture medium. While the experimental groups were added into different concentrations of r(?)tenone, which the final dose w(?) 0 , 1.0, 2.0 and 3.0 μmol/L respectively. They were continuously incubated for 24 h. The culture medium were discarded and then loaded with 1 μmol/L dihydrorhodamine fluorescent probe. Rotenone induced ROS production in PC 12 cells were measured using flow cytometry. The cells treatmented with the equal doses of rotenone for 24 h. After rhodanmine labeled, mitochondrial transmembrane potential was detected by laser scanning confocal microscope and the changes of mitochondrial ultrastructure induced by low, middle, and high doses of rotenone were observed under transmission electron microscope.Results: Treatment with 0.1 μmol/L, 1.0 μmol/L, 2.0 μmol/L and 3.0 μ mol/L concentrations of rotenone might increase the generation of ROS in PC12 cells, the value of fluorescence intensity as 1.55 ± 0.17 , 2.16 ± 0.10, 1.77 ± 0.20 and 1.41±0.12. The same rotenone might disrupt the...