Study The Relationship Between Typing Of Diagnosis Based On An Overall Analysis Of Signs And Symptoms Of AD In TCM And HLA-DR Gene

Objective To confirm the basic type of syndrome based on an overall analysis of signs and symptoms of AD in TCM and to investigate the relationship between HLA-DR genes and the type of syndrome of AD.Methods We confirm the basic type of syndrome of AD based on an overall analysis of signs and symptoms in TCM by reviewing literature and collection of clinic data.We used Polymerase chain reaction-Sequence specific primer technique to type HLA-DR sub-region in patients with AD and matched control subjects of healthy, unrelated in blood relationship of Han nationality from Guangdong province, to study the relationship between typing of diagnosis based on an overall analysis of signs and symptoms of AD in TCM and HLA-DR gene.We also observe blood serum total IgE, ALT/AST,BUN/Cr and the microcirculation in nail wrinkle in patients with AD and control subjects, to study the relationship between typing of diagnosis based on an overall analysis of signs and symptoms of AD in TCM and blood serum total IgE, ALT/AST,BUN/Cr. The severe scoring of atopic dermatitis was also observed in patients with AD.Results The allele frequencies of DRB1*15, DR7 were higher in AD patients than those in normal controls (P<0.05) .The allele frequency of DR7 was significantly higher in members with the type of accumulated of damp-heat in the skin than those in the other two types. The blood serum total IgE were higher in AD patients than those in normal controls ( P<0.05) the type of accumulated of damp-heat in the skin was the highest. the score of SCORAD were also significantly difference in all types. The result of the microcirculation in nail wrinkle in patients with AD were abnormity.Conclusion The basic type of syndrome of AD based on an overall analysis of signs and symptoms in TCM include the type of accumulated of wind-witness evil in theskin, the accumulated of damp-heat in the skin and the type of blood deficiency and wind-dryness. The result indicated the blood serum total IgE and the score of SCORAD can be looked as the judgment marker of the type of accumulated of damp-heat in the skin. The result also indicated The allele of DRB1*15? DR7 probably are the predisposing genes of AD and the DR7 genes probably is the predisposing genes of the type of accumulated of damp-heat in the skin. Above index can be used as the objective index of differentiation of symptoms and signs for classification syndrome of AD.